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KMID : 0357419940240010071
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Korean journal of Virology
1994 Volume.24 No. 1 p.71 ~ p.76
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Detection of Human Cytomegalovirus in Urine Sample of Infant by Polymerase Chain Reaction
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최철석
김은아/이경옥/이규범/이연태
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Abstract
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Polymerase Chain Reaction(PCR) was used to detect human cytomegalovirus(HCMV) in urine sample of an infant with neonatal hepatitis. The patient was HCMV seropositive for IgG, IgM by ELISA, and also showed high level of
aminotransferase(≥500I.U.)
and
direct bilirubine (>10mg/dl). Several reports have described difficulties of PCR in urine samples. This report is a rapid and reliable detection method of HCMV DNA in urine from a patient by extraction with guanidinium thiocyanate/silica, This
method is
based on lysing and adsorption of DNA for PCR amplification. Urine sample prepared by this method should good result. In our opinion, PCR with this method is sensitive, rapid and reliable procedure for successful PCR for detection of HCMV in
urine
sample.
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KEYWORD
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